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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been noticed in many tumors, which results in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but specific nucleotide sequence details is not nevertheless accessible.

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Blocking peptides are peptides that bind especially into the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified through the antibody.

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This manual outlines additional feasible optimization of mobile and immunoassay parameters to guarantee the absolute best benefits are...

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This guideline outlines additional attainable optimization of cellular and immunoassay parameters to make certain the absolute best results are...

Antibodies certain to the blocking peptide no more bind towards the epitope within the goal protein. By comparing the staining from your blocked antibody versus the antibody by yourself, you can see which staining is precise.

Focus on Facts Cyclin E belongs for the highly conserved cyclin household, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation designs which lead into the temporal coordination of each and every mitotic event. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is also degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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